5 Ligand-binding assay to measure the affinity and specificity of sterol-binding proteins in vitro / Rabih Darwiche and Roger Schneiter. Chemical pathways of corticosteroids, industrial synthesis from sapogenins / Ignacio Herráiz -- Genome sequencing of steroid-producing bacteria with illumina technology / Victoria Y. Shtratnikova, Mikhail I. Schelkunov, and Marina V. Donova -- Genome sequencing of steroid producing bacteria using ion torrent technology and a reference genome / Alberto Sola-Landa, Antonio Rodríguez-García, Carlos Barreiro, and Rosario Pérez-Redondo -- RNA-seq-based comparative transcriptomics : RNA preparation and bioinformatics / Antonio Rodríguez-García, Alberto Sola-Landa, and Carlos Barreiro -- Intra- and extra-cellular proteome analyses of steroid-producer mycobacteria / Carlos Barreiro, Alejandro Morales, Inés Vázquez-Iglesias, and Alberto Sola-Landa -- Genetic techniques for manipulation of the phytosterol biotransformation strain Mycobacterium neoaurum NRRL B-3805 / Jessica K. Loraine and Margaret C.M. Smith -- Identification and characterization of the genes and enzymes belonging to the bile acid catabolic pathway in Pseudomonas / José M. Luengo and Elías R. Olivera -- Selection of biodegrading phytosterol strains / María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal -- Stigmasterol removal by an aerobic treatment system / Soledad Chamorro, Claudia Xavier, Víctor Hernández, José Becerra, and Gladys Vidal -- Production and biotransformation of phytosterol microdispersions to produce 4-androstene-3,17-dione / Rodrigo A. Mancilla, Roberto Pavez-Díaz, and Alejandro Amoroso -- β-Sitosterol bioconversion to androstenedione in microtiter plates / Marco P.C. Marques and Pedro Fernandes -- Bioconversion of phytosterols into androstenedione by Mycobacterium / Kjell D. Josefsen, Anna Nordborg, and Håvard Sletta -- Scale-up of phytosterols bioconversion intoandrostenedione / Sonia Martínez-Cámara, Esther Bahíllo, José-Luis Barredo, and Marta Rodríguez-Sáiz -- Bioconversion of phytosterols into androstadienedione by Mycobacterium smegmatis CECT 8331 / Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García, and Beatriz Galán -- Process for biotransformation of androsta-4-ene-3,17-dione (4-AD) to androsta-1,4-diene-3,17-dione (ADD) / Surya Prakash and Abhay Bajaj -- Laboratory-scale hydroxylation of steroids by P450BM3 variants / Sabrina Hoebenreich, Martin Spinck, and Nathalie Nett -- Obtaining of 11α-hydroxyandrost-4-ene-3,17-dione from natural sterols / Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, and Marina V. Donova -- Steroid 11-alpha-hydroxylation by the fungi Aspergillus nidulans and Aspergillus ochraceus / Lidia Ortega-de los Ríos, José M. Luengo, and José M. Fernández-Cañón -- Biotransformation of DHEA into 7α, 15α-diOH-DHEA / Hui Li, Jin Sun, and Zhenghong Xu -- Stabilization of enzymes by using thermophiles / Ana Luisa Ribeiro, Mercedes Sánchez, Aurelio Hidalgo, and José Berenguer -- Biocatalysis of steroids with Mycobacterium sp. in aqueous and arganic media / Carla C.C.R. de Carvalho and Pedro Fernandes -- Downstream process snthesis for microbial steroids / Fabian B. Thygs and Juliane Merz -- Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC) / Govinda Guevara, Julián Perera, and Juana-María Navarro-Llorens -- Ligand-binding assay to measure the affinity and specificity of sterol-binding proteins in vitro / Rabih Darwiche and Roger Schneiter.
Summary:
This volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology.
Series:
Methods in molecular biology, 1064-3745 ; 1645 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.