Selection of error-less synthetic genes in yeast / Hisashi Hoshida, Tohru Yarimizu, and Rinji Akada. Synthetic gene design using codon optimization on-line (COOL) / Kai Yu, Kok Siong Ang, and Dong-Yup Lee -- Shuffle optimizer : a program to optimize DNA shuffling for protein engineering / John N. Milligan and Daniel J. Garry -- Simple cloning by prolonged overlap extension-PCR with application to the preparation of large-size random gene mutagenesis library in Escherichia coli / Chao Zhong [and others] -- SpeedyGenes : exploiting an improved gene synthesis method for the efficient production of synthetic protein libraries for directed evolution / Andrew Currin [and others] -- BASIC : a simple and accurate modular DNA assembly method / Marko Storch [and others] -- Enzymatic synthesis of single-stranded clonal pure oligonucleotides / Cosimo Ducani and Björn Högberg -- Rapid assembly of DNA via ligase cycling reaction (LCR) / Sunil Chandran -- PaperClip : a simple method for flexible multi-part DNA assembly / Maryia Trubitsyna [and others] -- Polymerase step reaction (PSR) method for gene and library synthesis / Brian S. DeDecker -- Clonetegration using OSIP plasmids : one-step DNA assembly and site-specific genomic integration in bacteria / Lun Cui and Keith E. Shearwin -- Generation of DNA constructs using the golden GATEway cloning method / Stephan Kirchmaier, Katharina Lust, and Jochen Wittbrodt -- Gene deletion by synthesis in yeast / Jinsil Kim, Dong-Uk Kim, and Kwang-Lae Hoe -- Efficient assembly of DNA using yeast homologous recombination (YHR) / Sunil Chandran and Elaine Shapland -- Simultaneous removal of multiple DNA Segments by polymerase chain reactions / Vishnu Krishnamurthy and Kai Zhang -- Rapid construction of recombinant plasmids by quickstep-cloning / Pawel Jajesniak and Tuck Seng Wong -- Immobilized MutS-mediated error removal of microchip-synthesized DNA / Wen Wan [and others] -- Selection of error-less synthetic genes in yeast / Hisashi Hoshida, Tohru Yarimizu, and Rinji Akada.
Summary:
This volume presents state-of-the art methods for the synthesis, design, assembly, post synthesis processing, and application of synthetic DNA to modern biotechnology. Chapters are divided into three general sections focusing on protocols for the computational design of synthetic DNA sequences, the synthesis, assembly and cloning of synthetic DNA, and post-synthesis error reduction strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic DNA: Methods and Protocols aims to help researchers further their research on manipulate DNA sequences.
Series:
Methods in molecular biology, 1064-3745 ; 1472 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.