27 records matched your query       

Title:

Protocols in molecular parasitology / edited by John E. Hyde.

Publisher:

Humana Press,

Copyright Date:

c1993

Description:

xiv, 470 p. : ill. ; 23 cm.

Subject:

Molecular parasitology--Congresses.
Parasites--laboratory manuals.--laboratory manuals.
Molecular Biology--methods.
Parasitology--methods.

Other Authors:

Hyde, John E.

Notes:

Includes bibliographical references and index.

Contents:

(cont) Antibody select procedure for characterization of expression clones -- Isolation of parasite genes using synthetic oligonucleotides -- Chromosome mapping methods for parasitic protozoa -- Transfection of Leishmania and Trypanosoma brucei by electroporation -- Two-dimensional polyacrylamide gel electrophoresis -- Isoenzyme electrophoresis for parasite characterization -- Surface labeling of schistosomes -- Monclonal antibody affinity chromotography -- Immunoblotting and enzyme linked immunosorbent assay -- Immunofluorescence of parasites -- Immunoelectron microscopic localization of antigens in malaria parasites.
(cont) Extraction and purification of nucleic acids from schistosomes -- Application of DNA and whole organisms to filter supports for DNA probe analysis: dot, slot, and touch blotting -- Development and use of repetitive sequences as DNA probes for parasite detection and species identification -- Analysis of the DNA of parasitic protozoa by flow cytometry -- PCR for low-level detection of malaria parasites in blood -- PCR methods for identification of point mutations and gene rearrangements -- Antigenic typing of field isolates of Plasmodium by DNA techniques -- Ribosomal RNA probes for detection and identification of species -- Rapid sequencing of parasite rRNA -- Antibody screening of expression libraries.
(cont) Preparation of blotted membrane for protein microsequencing -- Identification of T-cell epitopes.
Culturing and biological cloning of Trypanosoma brucei -- Culturing and biological cloning of Trypanosoma cruzi -- In vitro cultivation and biological cloning of Leishmania -- Simple in vitro cultivation of the malaria parasite Plasmodium falciparum (erythrocytic stages) suitable for large-scale preparations -- Synchronization and cloning of malaria parasites -- Culture and preparation of gametocytes of Plasmodium falciparum for immunochemical, molecular, and mosquito infectivity studies -- Culture of Schistosoma mansoni and production of life cycle stages -- Preparation of DNA and RNA from Trypanosoma brucei -- Isolation of RNA and DNA from Trypanosoma cruzi -- Isolation of DNA and RNA from Leishmania -- Extraction and purification of DNA and RNA from in vitro cultures of the malaria parasite Plasmodium falciparum.

Series:

Methods in molecular biology ; 21

ISBN:

9780896032392
0896032396

OCLC:

(OCoLC)28215171
(OCoLC)28220051

LCCN:

93008540

Locations:

USUX851 -- Iowa State University - Parks Library (Ames)

OVUX522 -- University of Iowa Libraries (Iowa City)