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Title:

Xenopus protocols : post-genomic approaches / edited by Stefan Hoppler, Peter D. Vize.

Edition:

2nd ed.

Publisher:

Humana Press ;

Copyright Date:

c2012

Description:

xiii, 568 p. : ill. (some col.) ; 26 cm.

Subject:

Xenopus--Embryology--Laboratory manuals.

Other Authors:

Hoppler, Stefan, 1965-
Vize, Peter D.

Notes:

Includes bibliographical references and index.

Contents:

Chemical genetics and drug discovery in Xenopus / Matthew L. Tomlinson, Adam E. Hendry, and Grant N. Wheeler -- Husbandry of Xenopus tropicalis / Alan Jafkins ... [et al.] -- Generating diploid embryos from Xenopus tropicalis / Florencia del Viso and Mustafa Khokha -- Navigating the Xenopus tropicalis genome / Ira L. Blitz -- Genetic analysis of Xenopus tropicalis / Timothy J. Geach, Derek L. Stemple, and Lyle B. Zimmerman -- Forward genetic screens in Xenopus using transposon-mediated insertional mutagenesis / Donald A. Yergeau ... [et al.] -- Targeted gene disruption with engineered zinc-finger nucleases (ZFNs) / John J. Young and Richard M. Harland -- Reverse genetic studies using antisense morpholino oligonucleotides / Yanan Zhao, Shoko Ishibashi, and Enrique Amaya -- Chemical genetics and drug discovery in Xenopus / Matthew L. Tomlinson, Adam E. Hendry, and Grant N. Wheeler --
Chromatin immunoprecipitation analysis of Xenopus embryos / Daniel R. Buchholz -- Generating transgenic frog embryos by restriction enzyme mediated integration (REMI) / Shoko Ishibashi, Kristen L. Kroll, and Enrique Amaya -- Simple method of transgenesis using I-SceI meganuclease in Xenopus / Shoko Ishibashi, Nick R. Love, and Enrique Amaya -- Using [phi]C31 integrase to mediate insertion of DNA in Xenopus embryos / You E. Li, Bryan G. Allen, and Daniel L. Weeks -- Xenopus transgenics : methods using transposons / Clair M. Kelley ... [et al.] -- Comparative genomics-based identification and analysis of cis-regulatory elements / Hajime Ogino ... [et al.] -- Tet-On binary systems for tissue-specific and inducible transgene expression / Daniel R. Buchholz -- Chromatin immunoprecipitation analysis of Xenopus embryos /
Boyan Bonev and Nancy Papalopulu -- Bromodeoxyuridine (BrdU) based protocol for characterizing proliferating progenitors in Xenopus embryos / Dasfne Lee-Liu ... [et al.] -- Databases of gene expression in Xenopus development / Michael J. Gilchrist and Nicolas Pollet -- Investigating alternative RNA splicing in Xenopus / Agnes Mereau and Serge Hardy -- Immunoisolation of protein complexes from Xenopus / Frank L. Conlon ... [et al.] -- Complementary proteomic analysis of protein complexes / Todd M. Greco ... [et al.] -- Antibody development and use in chromogenic and fluorescent immunostaining / Eamon Dubaissi ... [et al.] -- Multiple fluorescent in situ mRNA hybridization (FISH) on whole mounts and sections / Robert Lea ... [et al.] -- Methods to analyze microRNA expression and function during Xenopus development / Boyan Bonev and Nancy Papalopulu -- Bromodeoxyuridine (BrdU) based protocol for characterizing proliferating progenitors in Xenopus embryos /
Jeff Bowes. On-line resources for Xenopus / Sagar D. Joshi, Hye Young Kim, and Lance A. Davidson -- Mathematical modeling of gene regulatory networks in Xenopus development / Yasushi Saka -- Stem-cell-like embryonic explants to study cardiac development / Boni A. Afouda -- Studying regeneration in Xenopus / Caroline W. Beck -- On-line resources for Xenopus / Jeff Bowes.

Series:

Methods in molecular biology, 1064-3745 ; 917
Springer protocols

ISBN:

1617799912 (alk. paper)
9781617799914 (alk. paper)

OCLC:

(OCoLC)785074480

LCCN:

2012943830

Locations:

OVUX522 -- University of Iowa Libraries (Iowa City)