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05648aam a2200397 i 4500 001 2BA48DCC08B111E8A8C4D83297128E48 003 SILO 005 20180203010326 008 161104t20172017nyua b 001 0deng d 010 $a 2017931659 020 $a 1493968297 020 $a 9781493968299 035 $a (OCoLC)962230999 040 $a BTCTA $b eng $e rda $c BTCTA $d YDX $d IDL $d CHVBK $d NUI $d UtOrBLW $d SILO 245 00 $a Auxins and cytokinins in plant biology : $b methods and protocols / $c edited by Thomas Dandekar, Muhammad Naseem. 264 1 $a New York : $b Humana Press, $c [2017] 300 $a x, 204 pages : $b illustrations (some color) ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1569 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 505 00 $t High-throughput protoplast trans-activation (PTA) screening to define transcription factors in auxin-mediated gene regulation / $r Nora Wehner, JoÌrn Herfert, Wolfgang DroÌge-Laser, and Christoph Weiste. $t Analytical determination of auxins and cytokinins / $r Petre I. Dobrev, KlaÌra HoyerovaÌ, and Jan PetraÌsÌek -- $t Manipulation of auxin and cytokinin balance during the Plasmodiophora brassicae-Arabidopsis thaliana interaction / $r Jutta Ludwig-MuÌller, Susann Auer, Sabine JuÌlke, and Sabine Marschollek -- $t Microbial manipulation of auxins and cytokinins in plants / $r Anwar Hussain, Ihsan Ullah, and Shahida Hasnain -- $t Standardized method to assess infection rates of root-knot and cyst nematodes in Arabidopsis thaliana mutants with alterations in root development related to auxin and cytokinin signaling / $r RociÌo Olmo, Ana ClaÌudia Silva, Fernando E. DiÌaz-Manzano, Javier Cabrera, Carmen Fenoll, and Carolina Escobar-- $t Reconstruction of an immune dynamic model to simulate the contrasting role of auxin and cytokinin in plant immunity / $r Martin Kaltdorf, Thomas Dandekar, and Muhammad Naseem -- $t Interplay between auxin and cytokinin and its impact on mitogen activated protein kinase (MAPK) / $r Pallavi Singh and Alok Krishna Sinha -- $t Quantification of cytokinin levels and responses in abiotic stresses / $r Alfonso Albacete -- $t Assessment of cytokinin-induced immunity through quantification of Hyaloperonospora arabidopsidis infection in Arabidopsis thaliana / $r Ruth A. Watson and Cristiana T. Argueso -- $t Real-time genetic manipulations of the cytokinin pathway : a tool for laboratory and field studies / $r Martin SchaÌfer and Stefan Meldau -- $t Modulating the levels of plant hormone cytokinins at the host-pathogen interface / $r Muhammad Naseem, Shabana Shams, and Thomas Roitsch -- $t Analyzing cytokinin responses during plant-nematode interactions / $r Florian M.W. Grundler and Shahid Siddique -- $t Examining H2O2 Production in Arabidopsis leaves upon challenge by cytokinin / $r Shahjahan Shabbir Ahmed, Ihsan Ullah, Shazia Irfan, and Nazeer Ahmed -- $t Systems biology methodology combining transcriptome and interactome datasets to assess the implications of cytokinin signaling for plant immune networks / $r Meik Kunz, Thomas Dandekar, and Muhammad Naseem -- $t Monitoring of crosstalk between jasmonate and auxin in the framework of plant stress responses of roots / $r ViÌctor Carrasco Loba, Marta-Marina PeÌrez Alonso, and Stephan Pollmann -- $t High-throughput protoplast trans-activation (PTA) screening to define transcription factors in auxin-mediated gene regulation / $r Nora Wehner, JoÌrn Herfert, Wolfgang DroÌge-Laser, and Christoph Weiste. 520 $a This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins. 650 0 $a Auxin. $0 http://id.loc.gov/authorities/subjects/sh85010481 650 0 $a Cytokinins. $0 http://id.loc.gov/authorities/subjects/sh85035238 650 2 $a Indoleacetic Acids. $0 https://id.nlm.nih.gov/mesh/D007210 650 2 $a Cytokinins. $0 https://id.nlm.nih.gov/mesh/D003583 650 2 $a Plant Growth Regulators. $0 https://id.nlm.nih.gov/mesh/D010937 655 7 $a Laboratory manuals. $2 lcgft $0 http://id.loc.gov/authorities/genreForms/gf2014026120 700 1 $a Dandekar, Thomas, $d 1960- $e editor. $0 http://id.loc.gov/authorities/names/n97803682 700 1 $a Naseem, Muhammad, $e editor. $0 http://id.loc.gov/authorities/names/no2004043455 773 18 $w 990001282000202771 $g v:1569 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1569. $x 1064-3745 $0 http://id.loc.gov/authorities/names/n92002874 830 0 $a Springer protocols (Series). $x 1949-2448 $0 http://id.loc.gov/authorities/names/no2008180772 941 $a 1 952 $l OVUX522 $d 20180710092040.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=2BA48DCC08B111E8A8C4D83297128E48Initiate Another SILO Locator Search