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05555aam a2200337 a 4500 001 09F1B8D0019F11E89C78220097128E48 003 SILO 005 20180125010234 008 160312t20162016nyua b 001 0deng d 020 $a 1493937847 020 $a 9781493937844 035 $a (OCoLC)944473375 040 $a BTCTA $b eng $c BTCTA $d YDXCP $d YDX $d OCLCO $d OCLCF $d COD $d OCL $d NUI $d UtOrBLW $d SILO 245 00 $a Multipotent stem cells of the hair follicle : $b methods and protocols / $c edited by Robert M. Hoffman. 264 1 $a New York : $b Humana Press : $c [2016] 300 $a xi, 180 pages : $b color illustrations ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1453 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 520 $a This volume discusses methods for the study of multipotent and pluripotent stem cells of the hair follicle. The stem cells described are involved in both the growth of the hair follicle and its production of the hair shaft, as well as the growth of the hair follicle sensory nerve. Multipotent Stem Cells of the Hair Follicle: Methods and Protocols also explores very unexpected results such as that of the hair follicle-associated-pluripotent (HAP) stem cells, which not only have the capability for regenerating the hair follicle sensory nerve, but also can differentiate ex vivo and in vivo to multiple cell types not associated with the hair folliclethese include glial cells, motor neurons, and beating cardiac muscle cells. The potential for HAP stem cells for regenerative medicine is also discussed in detail. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Multipotent Stem Cells of the Hair Follicle: Methods and Protocols is a valuable resource for researchers interested in this field. 650 0 $a Stem cells $v Laboratory manuals. 650 0 $a Hair follicles $v Laboratory manuals. 655 2 $a Laboratory Manuals. $0 https://id.nlm.nih.gov/mesh/D020484 700 1 $a Hoffman, Robert M., $d 1944- $e editor. $0 http://id.loc.gov/authorities/names/no2008069235 773 18 $w 990001282000202771 $g v:1453 880 00 $6 505-00 $t Protocols for cryopreservation of intact hair follicle that maintain pluripotency of nestin-expressing hair-follicle-associated pluripotent (HAP) stem cells / $r Satoshi Kajiu ... [et al.]. $t Nestin-based reporter transgenic mouse lines / $r John Mignone, Natalia Peunova, and Grigori Enikolopov -- $t Discovery of HAP stem cells / $r Lingna Li and Robert M. Hoffman -- $t Peripheral-nerve and spinal-cord regeneration in mice using hair-follicle-associated pluripotent (HAP) stem cells / $r Yasuyuki Amoh, Kensei Katsuoka, and Robert M. Hoffman -- $t Construction of tissue-engineered nerve conduits seeded with neurons derived from hair-follicle neural crest stem cells / $r Fang Liu, Haiyan Lin, and Chuansen Zhang -- $t Nestin-expressing hair-follicle-associated pluripotent (HAP) stem cells promote whisker sensory-nerve growth in long-term 3D-Gelfoam® histoculture / $r Sumiyuki Mii ... [et al.] -- $t Isolation and culture of neural crest stem cells from human hair follicles / $r Ruifeng Yang and Xiaowei Xu -- $t Isolation of mouse hair follicle bulge stem cells and their functional analysis in a reconstitution assay / $r Ying Zheng ... [et al.] -- $t Hair follicle regeneration by transplantation of a bioengineered hair follicle germ / $r Katsunari Tezuka, Koh-ei Toyoshima, and Takashi Tsuji -- $t Hair induction by cultured mesenchymal cells using sphere formation / $r Kazuo Kishi and Ruka Hayashi -- $t Stereological quantification of cell cycle kinetics and mobilization of epithelial stem cells during wound healing / $r Eduardo MartiÌnez-MartiÌnez ... [et al.] -- $t Culture of dermal papilla cells from ovine wool follicles : an in vitro model for papilla size determination / $r Nicholas W. Rufaut, Allan J. Nixon, and Rodney D. Sinclair -- $t Isolation and fluorescence-activated cell sorting of mouse keratinocytes expressing β-galactosidase / $r Maria Kasper, Rune ToftgaÌrd, and Viljar Jaks -- $t Ectopic hair growth from transplanted whisker follicles on the spinal cord of mice / $r Wenluo Cao ... [et al.] -- $t Protocols for Gelfoam® histoculture of hair-shaft-producing mouse whisker follicles containing nestin-GFP-expressing hair-follicle-associated pluripotent (HAP) stem cells for long time periods / $r Wenluo Cao ... [et al.] -- $t Protocols for efficient differentiation of hair follicle-associated pluripotent (HAP) stem cells to beating cardiac muscle cells / $r Masateru Yashiro ... [et al.] -- $t Highly efficient neural differentiation of CD34-positive hair-follicle-associated pluripotent stem cells induced by retinoic acid and serum-free medium / $r Mohsen Sagha and Nowruz Najafzadeh -- $t Protocols for cryopreservation of intact hair follicle that maintain pluripotency of nestin-expressing hair-follicle-associated pluripotent (HAP) stem cells / $r Satoshi Kajiu ... [et al.]. 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1453. $x 1064-3745 $0 http://id.loc.gov/authorities/names/n92002874 830 0 $a Springer protocols (Series). $x 1949-2448 $0 http://id.loc.gov/authorities/names/no2008180772 941 $a 1 952 $l OVUX522 $d 20180710080419.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=09F1B8D0019F11E89C78220097128E48Initiate Another SILO Locator Search