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05965aam a2200349 i 4500 001 C22A4EEAFAD211E7A45C351A97128E48 003 SILO 005 20180116092551 008 170501t20172017nyua b 001 0deng d 020 $a 1493971824 020 $a 9781493971824 035 $a (OCoLC)985082407 040 $a BTCTA $b eng $c BTCTA $d BDX $d YDX $d STF $d GZM $d NUI $d SILO 245 00 $a Microbial steroids : $b methods and protocols / $c editors, JoseÌ-Luis Barredo, Ignacio HerraÌiz. 264 1 $a New York, NY : $b Humana Press, $c [2017] 300 $a xi, 373 pages : $b illustrations (some color) ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1645 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 520 $a This volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology. 505 00 $6 5 $t Ligand-binding assay to measure the affinity and specificity of sterol-binding proteins in vitro / $r Rabih Darwiche and Roger Schneiter. $t Chemical pathways of corticosteroids, industrial synthesis from sapogenins / $r Ignacio HerraÌiz -- $t Genome sequencing of steroid-producing bacteria with illumina technology / $r Victoria Y. Shtratnikova, Mikhail I. Schelkunov, and Marina V. Donova -- $t Genome sequencing of steroid producing bacteria using ion torrent technology and a reference genome / $r Alberto Sola-Landa, Antonio RodriÌguez-GarciÌa, Carlos Barreiro, and Rosario PeÌrez-Redondo -- $t RNA-seq-based comparative transcriptomics : RNA preparation and bioinformatics / $r Antonio RodriÌguez-GarciÌa, Alberto Sola-Landa, and Carlos Barreiro -- $t Intra- and extra-cellular proteome analyses of steroid-producer mycobacteria / $r Carlos Barreiro, Alejandro Morales, IneÌs VaÌzquez-Iglesias, and Alberto Sola-Landa -- $t Genetic techniques for manipulation of the phytosterol biotransformation strain Mycobacterium neoaurum NRRL B-3805 / $r Jessica K. Loraine and Margaret C.M. Smith -- $t Identification and characterization of the genes and enzymes belonging to the bile acid catabolic pathway in Pseudomonas / $r JoseÌ M. Luengo and EliÌas R. Olivera -- $t Selection of biodegrading phytosterol strains / $r MariÌa-AngeÌlica Mondaca, Maricel Vidal, Soledad Chamorro, and Gladys Vidal -- $t Stigmasterol removal by an aerobic treatment system / $r Soledad Chamorro, Claudia Xavier, ViÌctor HernaÌndez, JoseÌ Becerra, and Gladys Vidal -- $t Production and biotransformation of phytosterol microdispersions to produce 4-androstene-3,17-dione / $r Rodrigo A. Mancilla, Roberto Pavez-DiÌaz, and Alejandro Amoroso -- $t β-Sitosterol bioconversion to androstenedione in microtiter plates / $r Marco P.C. Marques and Pedro Fernandes -- $t Bioconversion of phytosterols into androstenedione by Mycobacterium / $r Kjell D. Josefsen, Anna Nordborg, and HaÌvard Sletta -- $t Scale-up of phytosterols bioconversion intoandrostenedione / $r Sonia MartiÌnez-CaÌmara, Esther BahiÌllo, JoseÌ-Luis Barredo, and Marta RodriÌguez-SaÌiz -- $t Bioconversion of phytosterols into androstadienedione by Mycobacterium smegmatis CECT 8331 / $r Julia GarciÌa-FernaÌndez, Igor MartiÌnez, Lorena FernaÌndez-CabezoÌn, Carmen Felpeto-Santero, JoseÌ-Luis GarciÌa, and Beatriz GalaÌn -- $t Process for biotransformation of androsta-4-ene-3,17-dione (4-AD) to androsta-1,4-diene-3,17-dione (ADD) / $r Surya Prakash and Abhay Bajaj -- $t Laboratory-scale hydroxylation of steroids by P450BM3 variants / $r Sabrina Hoebenreich, Martin Spinck, and Nathalie Nett -- $t Obtaining of 11α-hydroxyandrost-4-ene-3,17-dione from natural sterols / $r Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, and Marina V. Donova -- $t Steroid 11-alpha-hydroxylation by the fungi Aspergillus nidulans and Aspergillus ochraceus / $r Lidia Ortega-de los RiÌos, JoseÌ M. Luengo, and JoseÌ M. FernaÌndez-CanÌoÌn -- $t Biotransformation of DHEA into 7α, 15α-diOH-DHEA / $r Hui Li, Jin Sun, and Zhenghong Xu -- $t Stabilization of enzymes by using thermophiles / $r Ana Luisa Ribeiro, Mercedes SaÌnchez, Aurelio Hidalgo, and JoseÌ Berenguer -- $t Biocatalysis of steroids with Mycobacterium sp. in aqueous and arganic media / $r Carla C.C.R. de Carvalho and Pedro Fernandes -- $t Downstream process snthesis for microbial steroids / $r Fabian B. Thygs and Juliane Merz -- $t Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC) / $r Govinda Guevara, JuliaÌn Perera, and Juana-MariÌa Navarro-Llorens -- $t Ligand-binding assay to measure the affinity and specificity of sterol-binding proteins in vitro / $r Rabih Darwiche and Roger Schneiter. 650 0 $a Steroids $v Laboratory manuals. 650 2 $a Steroids. 655 7 $a Laboratory manuals. $2 lcgft 700 1 $a Barredo, JoseÌ-Luis, $e editor. 700 1 $a HerraÌiz, Ignacio, $e editor. 773 18 $w 990001282000202771 $g v:1645 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v 1645, $x 1064-3745 830 0 $a Springer protocols (Series), $x 1949-2448 941 $a 1 952 $l OVUX522 $d 20180710072032.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=C22A4EEAFAD211E7A45C351A97128E48Initiate Another SILO Locator Search