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05108aam a2200397 i 4500 001 DC4D268A776911E3809E827FDAD10320 003 SILO 005 20140107010125 008 130607t20132014nyua b 000 0 eng d 010 $a 2013946845 020 $a 9781627036542 020 $a 1627036547 035 $a (OCoLC)847348526 040 $a YDXCP $e rda $c YDXCP $d BTCTA $d IDL $d OCLCO $d STF $d NUI $d SILO 245 00 $a Brain development : $b methods and protocols / $c edited by Simon G. Sprecher. 264 1 $a New York : $b Humana Press, $c [2013] 300 $a x, 317 pages : $b illustrations (some colored) ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1082 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 505 00 $t Dye coupling and immunostaining of astrocyte-like glia following intracellular injection of fluorochromes in brain slices of the grasshopper, Schistocerca gregaria / $r George Boyan and Yu Liu -- $t Non-fluorescent RNA in situ hybridization combined with antibody staining to visualize multiple gene expression patterns in the embryonic brain of drosophila / $r David Jussen and Rolf Urbach -- $t Analysis of complete neuroblast cell lineages in the drosophila embryonic brain via DiI labeling / $r Karoline F. Kraft and Rolf Urbach -- $t Flybow to dissect circuit assembly in the Drosophila brain / $r Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker -- $t Immunofluorescent labeling of neural stem cells in the Drosophila optic lobe / $r Benjamin Perruchoud and Boris Egger -- $t Using MARCM to study drosophila brain development / $r Gudrun Viktorin -- $t Dye coupling and immunostaining of astrocyte-like glia following intracellular injection of fluorochromes in brain slices of the grasshopper, Schistocerca gregaria / $r George Boyan and Yu Liu -- 505 80 $t Transplantation of neural tissue : quail-chick chimeras / $r Richard P. Tucker and Qizhi Gong -- $t In Situ Hybridization and Immunostaining of Xenopus Brain / $r Kai-li Liu ... [et al.] -- $t Microinjection manipulations in the elucidation of xenopus brain development / $r Cristine Smoczer ... [et al.] -- $t Morpholino studies in xenopus brain development / $r Jennifer E. Bestman and Hollis T. Cline -- $t Sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification / $r Gilbert Lauter, Iris SoÌll, and Giselbert Hauptmann -- $t Dynamic neuroanatomy at subcellular resolution in the zebrafish / $r AdeÌle Faucherre and HernaÌn LoÌpez-Schier -- $t Anatomical dissection of zebrafish brain development / $r Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins -- $t Immunohistochemistry and in situ hybridization in the developing chicken brain / $r Richard P. Tucker and Qizhi Gong -- $t Transplantation of neural tissue : quail-chick chimeras / 505 80 $r Claudius F. Kratochwil and Filippo M. Rijli. $t Cre/Lox system to assess the development of the mouse brain / $r Irwin Andermatt and Esther T. Stoeckli -- $t Immunohistochemistry and RNA in situ hybridization in mouse brain development / $r Jinling Liu and Aimin Liu -- $t In utero electroporation to study mouse brain development / $r Emilie Pacary and François Guillemot -- $t Cre/Lox system to assess the development of the mouse brain / $r Claudius F. Kratochwil and Filippo M. Rijli. 520 $a Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species. 650 12 $a Brain $x growth & development $v Laboratory Manuals. $2 [MeSH] 650 22 $a Genes, Developmental $v Laboratory Manuals. $2 [MeSH] 650 22 $a Models, Animal $v Laboratory Manuals. $2 [MeSH] 650 0 $a Brain $x Growth $v Laboratory manuals. 655 7 $a Laboratory Manuals. $2 mesh 700 1 $a Sprecher, Simon G., $e editor. 710 2 $a Springer Science+Business Media, $e copyright holder. 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1082. 830 0 $a Springer protocols (Series) 941 $a 1 952 $l OVUX522 $d 20171220050954.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=DC4D268A776911E3809E827FDAD10320Initiate Another SILO Locator Search