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03625cam a2200409 a 4500 001 F1CEE83A2ADF11DEA2B9FC05A8D7520A 005 200904161840.0 008 930511s1993 njua b 001 0 eng c 010 $a 93008540 020 $a 9780896032392 020 $a 0896032396 035 $a (OCoLC)28215171 035 $a (OCoLC)28220051 040 $a DNLM/DLC $c DLC $d SILO $d TMV $d IWA $d SILO 060 00 $a W1 $b ME9616J v.21 1993 060 10 $a QX 25 P967 1993 082 00 $a 574.5/249/0724 $2 20 245 0 $a Protocols in molecular parasitology / $c edited by John E. Hyde. 260 $a Totowa, N.J. : $b Humana Press, $c c1993. 300 $a xiv, 470 p. : $b ill. ; $c 23 cm. 490 1 $a Methods in molecular biology ; $v 21 504 $a Includes bibliographical references and index. 505 0 $a (cont) Antibody select procedure for characterization of expression clones -- Isolation of parasite genes using synthetic oligonucleotides -- Chromosome mapping methods for parasitic protozoa -- Transfection of Leishmania and Trypanosoma brucei by electroporation -- Two-dimensional polyacrylamide gel electrophoresis -- Isoenzyme electrophoresis for parasite characterization -- Surface labeling of schistosomes -- Monclonal antibody affinity chromotography -- Immunoblotting and enzyme linked immunosorbent assay -- Immunofluorescence of parasites -- Immunoelectron microscopic localization of antigens in malaria parasites. 505 0 $a (cont) Extraction and purification of nucleic acids from schistosomes -- Application of DNA and whole organisms to filter supports for DNA probe analysis: dot, slot, and touch blotting -- Development and use of repetitive sequences as DNA probes for parasite detection and species identification -- Analysis of the DNA of parasitic protozoa by flow cytometry -- PCR for low-level detection of malaria parasites in blood -- PCR methods for identification of point mutations and gene rearrangements -- Antigenic typing of field isolates of Plasmodium by DNA techniques -- Ribosomal RNA probes for detection and identification of species -- Rapid sequencing of parasite rRNA -- Antibody screening of expression libraries. 505 0 $a (cont) Preparation of blotted membrane for protein microsequencing -- Identification of T-cell epitopes. 505 0 $a Culturing and biological cloning of Trypanosoma brucei -- Culturing and biological cloning of Trypanosoma cruzi -- In vitro cultivation and biological cloning of Leishmania -- Simple in vitro cultivation of the malaria parasite Plasmodium falciparum (erythrocytic stages) suitable for large-scale preparations -- Synchronization and cloning of malaria parasites -- Culture and preparation of gametocytes of Plasmodium falciparum for immunochemical, molecular, and mosquito infectivity studies -- Culture of Schistosoma mansoni and production of life cycle stages -- Preparation of DNA and RNA from Trypanosoma brucei -- Isolation of RNA and DNA from Trypanosoma cruzi -- Isolation of DNA and RNA from Leishmania -- Extraction and purification of DNA and RNA from in vitro cultures of the malaria parasite Plasmodium falciparum. 650 0 $a Molecular parasitology $x Congresses. 650 12 $a Parasites $x laboratory manuals. $x laboratory manuals. 650 22 $a Molecular Biology $x methods. 650 22 $a Parasitology $x methods. 700 1 $a Hyde, John E. 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v 21. 939 $a 1812577 941 $a 2 952 $l OVUX522 $d 20240117013621.0 952 $l USUX851 $d 20180703045559.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=F1CEE83A2ADF11DEA2B9FC05A8D7520AInitiate Another SILO Locator Search