Methods to assess the activation of the alternative (noncanonical) NF-κB pathway by non-death TNF receptors / Changying Jiang and Xin Lin -- Detection of IκB degradation dynamics and IκB-α ubiquitination / Petro Starokadomskyy and Ezra Burstein -- Measurement of NF-κB transcriptional activity and identification of NF-κB cis -regulatory elements using luciferase assays / Patricia E. Collins, Christine O'Carroll, and Ruaidhrí J. Carmody -- Assessing sites of NF-κB DNA binding using chromatin immunoprecipitation / Amy Colleran, Patricia E. Collins, and Ruaidhrí J. Carmody -- IKK kinase assay for assessment of canonical NF-κB activation in neurons / Anca B. Mihalas and Mollie K. Meffert -- Analysis of epidermal growth factor-induced NF-κB signaling / Changying Jiang and Xin Lin -- Methods to assess the activation of the alternative (noncanonical) NF-κB pathway by non-death TNF receptors / Anna D. Reichardt [and 3 others] -- TRAF protein function in noncanonical NF-κB signaling / Zhaoxia Qu and Gutian Xiao -- Noncanonical NF-κB activation and SDF-1 expression in human endothelial cells / Kelly A. McCorkell and Michael J. May -- Stable reconstitution of IKK-deficient mouse embryonic fibroblasts / Carolyn M. Gray and Michael J. May -- Dissecting NF-κB signaling induced by genotoxic agents via Genetic Complementation of NEMO-Deficient 1.3E2 Cells / Shawn S. Jackson and Shigeki Miyamoto -- Visualizing TCR-induced POLKADOTS formation and NF-κB activation in the D10 T-cell clone and mouse primary effector T cells / Suman Paul and Brian C. Schaefer -- Detection of recombinant and cellular MALT1 paracaspase activity / Daniel Nagel and Daniel Krappmann -- TRAF protein function in noncanonical NF-κB signaling / Anna D. Reichardt [and 3 others] -- Control of NF-κB subunits by ubiquitination / Robert A. Chong [and 3 others] -- Elucidating dynamic protein-protein interactions and ubiquitination in NF-κB signaling pathways / Noula Shembade and Edward W. Harhaj -- Immunoblot analysis of linear polyubiquitination of NEMO / Yoshiteru Sasaki [and 3 others] -- In vitro detection of NEMO-ubiquitin binding using DELFIA and microscale thermophoresis assays / Michelle Vincendeau , Daniel Krappmann , and Kamyar Hadian -- Use of fluorescence spectroscopy for quantitative investigations of ubiquitin interactions with the ubiquitin- binding domains of NEMO / Virginie Dubosclard, Elisabeth Fontan, and Fabrice Agou -- Generation of a proteolytic signal : E3/E2-mediated polyubiquitination of IκBα / Robert A. Chong [and 3 others] -- Control of NF-κB subunits by ubiquitination / Daniela S. Bassères and Albert S. Baldwin -- Immunohistochemical analysis of NF-κB in human tumor tissue / Haiying Li, Petro Starokadomskyy, and Ezra Burstein -- Using sequential immunoprecipitation and mass spectrometry to identify methylation of NF-κB / Tao Lu and George R. Stark -- Methods to detect NF-κB acetylation and methylation / JinJing Chen and Lin-Feng Chen -- Method for the quantitative analysis of stimulation- induced nuclear translocation of the p65 subunit of NF-κB from patient-derived dermal fibroblasts / Alex W. Wessel and Eric P. Hanson -- Methods for assessing the in vitro transforming activity of NF-κB transcription factor c-Rel and related proteins / Thomas D. Gilmore and Céline Gélinas -- Using RNA interference in lung cancer cells to target the IKK-NF-κB pathway / Daniela S. Bassères and Albert S. Baldwin -- Immunohistochemical analysis of NF-κB in human tumor tissue / Helena Shaked, Monica Guma, and Michael Karin -- Analysis of NF-κB activation in mouse intestinal epithelial cells / Anita Gaurnier-Hausser and Nicola J. Mason -- NEMO-binding domain peptide inhibition of inflammatory signal-induced NF-κB activation in vivo / Kelly A. McCorkell and Michael J. May -- Regulation of NF-κB signaling in osteoclasts and myeloid progenitors / Gaurav Swarnkar and Yousef Abu-Amer -- NF-κB activation with aging : characterization and therapeutic inhibition / Jing Zhao [and 4 others] -- "Sneaking-Ligand" approach : cell-type specific inhibition of the classical NF-κB pathway / Bettina Sehnert [and 3 others] -- Sneaking-ligand fusion proteins attenuate serum transfer arthritis by endothelium-targeted NF-κB inhibition / Bettina Sehnert [and 4 others] -- Analysis of NF-κB activation in mouse intestinal epithelial cells / Helena Shaked, Monica Guma, and Michael Karin -- Studying NF-κB signaling with mathematical models / Simon Mitchell, Rachel Tsui, and Alexander Hoffmann. Methods for analyzing the evolutionary relationship of NF-κB proteins using free, web-driven bioinformatics and phylogenetic tools / John R. Finnerty and Thomas D. Gilmore -- Studying NF-κB signaling with mathematical models / Simon Mitchell, Rachel Tsui, and Alexander Hoffmann.
Series:
Methods in molecular biology, 1064-3745 ; v. 1280 Springer protocols, 1949-2448
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