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05294aam a2200361 i 4500 001 873D4CD2EAD411E7BA71765497128E48 003 SILO 005 20171227010214 008 150831t20162016nyua b 001 0deng d 020 $a 9781493933938 020 $a 1493933930 020 $a 9781493933914 020 $a 1493933914 035 $a (OCoLC)919479594 040 $a YDXCP $b eng $c YDXCP $d BTCTA $d OCLCQ $d HNK $d OCLCF $d NUI $d UtOrBLW $d SILO 245 00 $a Biotechnology of plant secondary metabolism : $b methods and protocols / $c edited by Arthur Germano Fett-Neto. 264 1 $a New York : $b Humana Press, $c [2016] 300 $a xi, 180 pages : $b illustrations (some color) ; $c 27 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1405 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographical references and index. 505 00 $t Production of recombinant caffeine synthase from guarana (Paullinia cupana var. sorbilis) in Escherichia coli / $r FlaÌvia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera -- $t Stimulant paste preparation and bark streak tapping technique for pine oleoresin extraction / $r Thanise Nogueira FuÌller [and others] -- $t Modified protocol for high-quality RNA extraction from oleoresin-producing adult pines / $r JuÌlio CeÌsar de Lima [and others] -- $t Collection of apoplastic fluids from Arabidopsis thaliana leaves / $r Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier -- $t From plant extract to a cDNA encoding a glucosyltransferase candidate : proteomics and transcriptomics as tools to help elucidate saponin biosynthesis in Centella asiatica / $r Fernanda de Costa [and others] -- $t Production of recombinant caffeine synthase from guarana (Paullinia cupana var. sorbilis) in Escherichia coli / $r FlaÌvia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera -- 505 80 $t Analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants / $r IneÌs Carqueijeiro [and others] -- $t Production of aromatic plant terpenoids in recombinant baker's yeast / $r Anita Emmerstorfer-Augustin and Harald Pichler -- $t Purification of a recombinant polyhistidine-tagged glucosyltransferase using immobilized metal-affinity chromatography (IMAC) / $r Fernanda de Costa [and others] -- $t Western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes / $r Morten Egevang Jørgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier -- $t Laser capture microdissection : avoiding bias in analysis by selecting just what matters / $r MaÌrcia R. de Almeida and Martina V. StroÌmvik -- $t Analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants / $r IneÌs Carqueijeiro [and others] -- 505 80 $t Knockdown of polyphenol oxidase gene expression in potato (Solanum tuberosum L.) with artificial MicroRNAs / $r Ming Chi [and others]. $t Design, construction, and validation of artificial MicroRNA vectors using agrobacterium-mediated transient expression system / $r Basdeo Bhagwat [and others] -- $t Knockdown of polyphenol oxidase gene expression in potato (Solanum tuberosum L.) with artificial MicroRNAs / $r Ming Chi [and others]. 520 $a This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry. 650 0 $a Plant cells and tissues $v Laboratory manuals. 700 1 $a Fett-Neto, Arthur Germano, $d 1967- $e editor. $0 http://id.loc.gov/authorities/names/no2010094991 773 18 $w 990001282000202771 $g no:1405 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1405. $x 1064-3745 $0 http://id.loc.gov/authorities/names/n92002874 830 0 $a Springer protocols (Series). $x 1948-2448 $0 http://id.loc.gov/authorities/names/no2008180772 941 $a 1 952 $l OVUX522 $d 20171227033217.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=873D4CD2EAD411E7BA71765497128E48Initiate Another SILO Locator Search