Programmed cell death in plants : an overview / Vittoria Locato and Laura De Gara -- Investigation of morphological features of autophagy during plant programmed cell death / Alessio Papini -- Markers of developmentally regulated programmed cell death and their analysis in cereal seeds / Fernando Domínguez and Francisco Javier Cejudo -- Measurement of hypersensitive cell death triggered by avirulent bacterial pathogens in Arabidopsis / Zahra Imanifard, Elodie Vandelle, and Diana Bellin -- Immunity-associated programmed cell death as a tool for the identification of genes essential for plant innate immunity / Bangjun Zhou and Lirong Zeng -- Analysis of mitochondrial makers of programmed cell death / Dong Xiao, Huyi He, Wenjing Huang, Thet Lwin Oo, Aiqin Wang, and Long-Fei He -- Studying retrograde signaling in plants / Martyna Broda and Olivier Van Aken -- ROS and cell death in tomato roots infected by Meloidogyne incognita / Paola Leonetti -- Detection of reactive oxygen and nitrogen species (ROS/RNS) during hypersensitive cell death / Laura C. Terrón-Camero, Eliana Molina-Moya, María Sanz-Fernández, Luisa M. Sandalio, and María C. Romero-Puertas -- DNA diffusion assay applied to lant cells / Ance Macovei, Mattia Donà, Daniela Carbonera, and Alma Balestrazzi -- Analysis of reactive carbonyl species generated under oxidative stress / Jun'ichi Mano and Md. Sanaullah Biswas -- In vivo analysis of calcium levels and glutathione redox status in Arabidopsis epidermal leaf cells infected with the hypersensitive response-inducing bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB) / Fabrizio Gandolfo Doccula, Laura Luoni, Smrutisanjita Behera, Maria Cristina Bonza, and Alex Costa -- Measurement of cyclic GMP during plant hypersensitive disease resistance response / Jian Chen, Diana Bellin, and Elodie Vandelle -- Detection of MAPK3/6 phosphorylation during hpersensitive response (HR)-associated programmed cell death in plants / Qingyu Wu and David Jackson -- Measurement of the Caspase-1-like activity of vacuolar processing enzyme in plants / Noriyuke Hatsugai and Ikuko Hara-Nishimura -- Plant cell cultures as model systems to study programmed cell death / Sara Cimini, Maria Beatrice Ronci, Elisabetta Barizza, Maria Concetta de Pinto, Vittoria Locato, Fiorella Lo Schivo, and Laura De Gara.
Summary:
This detailed volume explores numerous protocols that can be specifically used for studying plant programmed cell death (PCD), a mechanism involved in a number of physiological and pathological processes that are triggered by developmental requirements as well as changing/adverse environmental conditions. Rich in methodological recommendations, this book enables readers to perform the described analyses, alerting them to possible difficulties and showing how the methods can also be applied in different contexts and/or on different plant matrices. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.uthoritative and practical, Plant Programmed Cell Death: Methods and Protocols provides essential methodological information that will help researchers to expand the field of plant PCD studies.
Series:
Methods in molecular biology, 1064-3745 ; 1743 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.