Dye coupling and immunostaining of astrocyte-like glia following intracellular injection of fluorochromes in brain slices of the grasshopper, Schistocerca gregaria / George Boyan and Yu Liu -- Non-fluorescent RNA in situ hybridization combined with antibody staining to visualize multiple gene expression patterns in the embryonic brain of drosophila / David Jussen and Rolf Urbach -- Analysis of complete neuroblast cell lineages in the drosophila embryonic brain via DiI labeling / Karoline F. Kraft and Rolf Urbach -- Flybow to dissect circuit assembly in the Drosophila brain / Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker -- Immunofluorescent labeling of neural stem cells in the Drosophila optic lobe / Benjamin Perruchoud and Boris Egger -- Using MARCM to study drosophila brain development / Gudrun Viktorin -- Dye coupling and immunostaining of astrocyte-like glia following intracellular injection of fluorochromes in brain slices of the grasshopper, Schistocerca gregaria / George Boyan and Yu Liu -- Transplantation of neural tissue : quail-chick chimeras / Richard P. Tucker and Qizhi Gong -- In Situ Hybridization and Immunostaining of Xenopus Brain / Kai-li Liu ... [et al.] -- Microinjection manipulations in the elucidation of xenopus brain development / Cristine Smoczer ... [et al.] -- Morpholino studies in xenopus brain development / Jennifer E. Bestman and Hollis T. Cline -- Sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification / Gilbert Lauter, Iris Söll, and Giselbert Hauptmann -- Dynamic neuroanatomy at subcellular resolution in the zebrafish / Adèle Faucherre and Hernán López-Schier -- Anatomical dissection of zebrafish brain development / Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins -- Immunohistochemistry and in situ hybridization in the developing chicken brain / Richard P. Tucker and Qizhi Gong -- Transplantation of neural tissue : quail-chick chimeras / Claudius F. Kratochwil and Filippo M. Rijli. Cre/Lox system to assess the development of the mouse brain / Irwin Andermatt and Esther T. Stoeckli -- Immunohistochemistry and RNA in situ hybridization in mouse brain development / Jinling Liu and Aimin Liu -- In utero electroporation to study mouse brain development / Emilie Pacary and François Guillemot -- Cre/Lox system to assess the development of the mouse brain / Claudius F. Kratochwil and Filippo M. Rijli.
Summary:
Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
Series:
Methods in molecular biology, 1064-3745 ; 1082 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.