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Title:
Mesenchymal stem cells : methods and protocols / edited by Massimiliano Gnecchi.
Edition:
Second edition.
Publisher:
HumanaPress,
Copyright Date:
2016
Description:
xix, 570 pages : illustrations (some color) ; 26 cm.
Subject:
Mesenchymal stem cells--Laboratory manuals.
Mesenchymal Stromal Cells.
Cell- and Tissue-Based Therapy--methods.
Other Authors:
Gnecchi, Massimiliano, editor. http://id.loc.gov/authorities/names/nb2016012908
Notes:
Includes bibliographical references and index.
Contents:
Mesenchymal Stromal Cells In Hematopoietic Stem Cell Transplantation -- Bone Tissue Engineering: Past {u2013} Present {u2013} Future -- Mesenchymal Stem Cells For Osteochondral Tissue Engineering -- Mesenchymal Stem Cells In Cardiology -- Mesenchymal Stem Cells In Kidney Repair -- Mesenchymal Stem Cells In Lipogems, A Reverse Story: From Clinical Practice To Basic Science -- Paracrine Mechanisms Of Mesenchymal Stem Cells In Tissue Repair -- Protocols For In Vitro Differentiation Of Human Mesenchymal Stem Cells Into Osteogenic, Chondrogenic And Adipogenic Lineages -- Colony Forming Unit Assays -- Methods and Strategies For Lineage Tracing Of Mesenchymal Progenitor Cells -- Isolation Of Mouse Bone Marrow Mesenchymal Stem Cells -- Isolation Of Pig Bone Marrow-Derived Mesenchymal Stem Cells -- Isolation, Culture, And Phenotypic Characterization Of Mesenchymal Stromal Cells From The Amniotic Membrane Of Human Term Placenta -- Isolation, Culture And Characterization Of Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells -- Isolation, Expansion And Immortalization Of Human Adipose-Derived Mesenchymal Stromal Cells From Biopsies And Liposuction Specimens -- Optimization Of Mesenchymal Stem Cells To Increase Their Therapeutic Potential -- Directed Differentiation Of Human Induced Pluripotent Stem Cells To Mesenchymal Stem Cells -- Isolation And Manufacture Of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells -- Quality Control Assays For Clinical Grade Human Mesenchymal Stromal Cells: Methods For ATMP Release -- Quality Control Assays For Clinical Grade Human Mesenchymal Stromal Cells: Validation Strategy -- Cryopreservation And Revival Of Human Mesenchymal Stromal Cells -- Clinical-Grade Manufacturing Of Therapeutic Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Culture Systems -- GMP-Compliant Expansion of Clinical Grade Human Mesenchymal Stromal/Stem Cells Using a Closed Hollow-Fibre Bioreacto -- Engineering Small-Scale and Scaffold-Based Bone Organs Via Endochondral Ossification Using Adult Progenitor Cells -- Fabrication Of Elasticity-Tunable Gelatinous Gel For Mesenchymal Stem Cell Culture -- Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium -- Tips on How to Collect and Administer the Mesenchymal Stem Cell Secretome for Central Nervous System Applications -- Soluble Factors from Human Fetal Bone Marrow-Derived Mesenchymal Stem Cells: Preparation of Conditioned Medium and its Effect On Tumor Cells -- Isolation and Characterization of Exosome From Human Embryonic Stem Cell-Derived Cmyc-Immortalized Mesenchymal Stem Cells -- Transcriptomic Analysis of Adult Renal Derived Mesenchymal Stem-Like Cells -- Proteomic Analysis of Mesenchymal Stem Cells -- Unraveling Mesenchymal Stem Cells{u2019}s Dynamic Secretome Through Non-Targeted Proteomics -- Identification Of Factors Produced And Secreted By Mesenchymal Stromal Cells With The SILAC Method.
Series:
Methods in molecular biology, 1064-3745 ; 1416
Springer protocols, 1949-2448
ISBN:
9781493935826
1493935828
OCLC:
(OCoLC)932095837
LCCN:
2016936658
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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