Constructing free energy landscapes of nucleic acid hairpin unfolding / Micah J. McCauley, Ioulia Rouzina, and Mark C. Williams. Synthesis of designer DNA / Rachel C. Little, Colette J. Whitfield, Eimer M. Tuite, and Andrew R. Pike -- Synthesis of DNA-based nanowires / Alexander Kotlyar -- Preparation of anti-miR PNAs for drug development and nanomedicine / Alex Manicardi, Roberto Gambari, Luisa de Cola, and Roberto Corradini -- Loading of PNA and other molecular payloads on inorganic nanostructures for theranostics / Alessandro Bertucci, Simone Silvestrini, Roberto Corradini, and Luisa De Cola -- Design and characterization of pH-triggered DNA nanoswitches and nanodevices based on DNA triplex structures / Andrea Idili and Francesco Ricci -- Characterizing surface-immobilized DNA structures and devices using a quartz crystal microbalance with dissipation monitoring (QCM-D) / Katherine E. Dunn, Martin A. Trefzer, Steven Johnson, and Andy M. Tyrrell -- Hybridization chain reaction design and biosensor implementation / Andrea Miti and Giampaolo Zuccheri -- Ultrasensitive detection of metal ions with DNA nanostructure / Mingshu Xiao, Xiangmeng Qu, Li Li, and Hao Pei -- Spatially resolved peptide-DNA nanoassemblages for biomarker detection : a synergy of DNA-directed immobilization and nanografting / Abimbola Feyisara Adedeji, Elena Ambrosetti, Loredana Casalis, and Matteo Castronovo -- LSPR detection of nucleic acids on nanoparticle monolayers / Sophie Thamm, Andrea Csaki, and Wolfgang Fritzsche -- DNA-templated silver nanoclusters for DNA methylation detection / Hanie Ahmadzade Kermani, Morteza Hosseini, and Mehdi Dadmehr -- Selection of structure-switching DNA aptamers binding soluble small molecules and SPR validation of enrichment / Enrico Tenaglia and Carlotta Guiducci -- Dielectrophoretic stretching of DNA / Eva-Maria Laux, Frank F. Bier, and Ralph Holzel -- Practical guide to molecular dynamics simulations of DNA origami systems / Jejoong Yoo, Chen-Yu Li, Scott Michael Slone, Christopher Maffeo, and Aleksei Aksimentiev -- Single-molecule patterning via DNA nanostructure assembly : a reusable platform / Da Huang, Mark Freeley, and Matteo Palma -- Directed protein adsorption through DNA origami masks / Saminathan Ramakrishnan, Guido Grundmeier, and Adrian Keller -- DNA origami structures interfaced to inorganic nanodevices / Claudia Dalmastri, Weihua Han, Stefano Vespucci, Liqian Wang, and Piero Morales -- Tuning gold nanoparticles plasmonic properties by DNA nanotechnology / Valentina Masciotti, Denys Naumenko, Marco Lazzarino, and Luca Piantanida -- DNA-assisted molecular lithography / Boxuan Shen, Veikko Linko, and J. Jussi Toppari -- Constructing free energy landscapes of nucleic acid hairpin unfolding / Micah J. McCauley, Ioulia Rouzina, and Mark C. Williams.
Summary:
This second edition provides new and updated methods that detail new DNA nanotechnology techniques. Chapters focus on DNA origami nanostructures for arranging matter in the nanoscale or on their manipulation with the aid of other technologies, on procedures for making nucleic acids nanostructures of different kinds, and methods to simulate complex nanostructures or to use them in biosensing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Nanotechnology: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Series:
Methods in molecular biology, 1064-3745 ; v. 1811 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.