Highly efficient and reliable DNA aptamer selection using the partitioning capabilities of ddPCR : the Hi-Fi SELEX method ./ Aaron Ang, Eric Ouellet, Karen C. Cheung, and Charles Haynes. Basic concepts and validation of digital PCR measurements / Leonardo Pinheiro and Kerry R. Emslie -- Fundamentals of counting statistics in digital PCR : I just measured two target copies-what does it mean? / Svilen Tzonev -- Control materials and digital PCR methods for evaluation of circulating cell-free DNA extractions from plasma / Alexandra S. Whale, Ana Fernandez-Gonzalez, Alice Gutteridge, and Alison S. Devonshire -- Multiplex droplet digital PCR protocols for quantification of GM maize events / David Dobnik, Bjørn Spilsberg, Alexandra Bogozalec Kosir, Dejan Stebih, Dany Morisset, Arne Holst-Jensen, and Jana Zel -- Using droplet digital PCR to detect coinfection of human herpesviruses 6A and 6B (HHV-6A and HHV-6B) in clinical samples / Ashley Vellucci, Emily C. Leibovitch, and Steven Jacobson -- Biomarkers in cerebrospinal fluid : analysis of cell-free circulating mitochondrial DNA by digital PCR / Petar Podlesniy and Ramon Trullas -- Testing of general and human-associated fecal contamination in waters / Yiping Cao, Meredith R. Raith, and John F. Griffith -- Analyzing copy number variation with droplet digital PCR / Avery Davis Bell, Christina L. Usher, and Steven A. McCarroll -- Assessing HER2 amplification in plasma cfDNA / Isaac Garcia-Murillas and Nicholas C. Turner -- Detection and quantification of mosaic genomic DNA variation in primary somatic tissues using ddPCR : analysis of mosaic transposable-element insertions, copy-number variants, and single-nucleotide variants / Bo Zhou, Michael S. Haney, Xiaowei Zhu, Reenal Pattni, Alexej Abyzov, and Alexander E. Urban -- Monitoring of response and resistance in plasma of EGFR-mutant lung cancer using droplet digital PCR / Yanan Kuang, Allison O'Connell, Adrian G. Sacher, Nora Feeney, Ryan S. Alden, Geoffrey R. Oxnard, and Cloud P. Paweletz -- Detection of cancer DNA in early stage and metastatic breast cancer patients / Arielle J. Medford, Riaz N. Gillani, and Ben Ho Park -- Droplet digital PCR for minimal residual disease detection in mature lymphoproliferative disorders / Daniela Drandi, Simone Ferrero, and Marco Ladetto -- Quantitation of JAK2 V617F allele burden by using the QuantStudio 3D digital PCR system / Elena Kinz and Axel Muendlein -- Novel multiplexing strategies for quantification of rare alleles using ddPCR / Miguel Alcaide and Ryan D. Morin -- Identification and use of personalized genomic markers for monitoring circulating tumor DNA / Yilun Chen, Anthony M. George, Eleonor Olsson, and Lao H. Saal -- Single color multiplexed ddPCR copy number measurements and single nucleotide variant genotyping / Christina M. Wood-Bouwens and Hanlee P. Ji -- Universal droplet digital PCR approach for monitoring of graft health after transplantation using a preselected SNP set / Julia Beck, Michael Oellerich, and Ekkehard Schutz -- Detection and quantification of HDR and NHEJ induced by genome editing at endogenous gene loci using droplet digital PCR / Yuichiro Miyaoka, Steven J. Mayerl, Amanda H. Chan, and Bruce R. Conklin -- DNA methylation analysis using droplet digital PCR / Ming Yu, Tai J. Heinzerling, and William M. Grady -- Simultaneous quantification of multiple alternatively spliced mRNA transcripts using droplet digital PCR / Bing Sun and Yun-Ling Zheng -- Using droplet digital PCR to analyze allele-specific RNA expression / Nolan Kamitaki, Christina L. Usher, and Steven A. McCarroll -- Very low abundance single-cell transcript quantification with 5-plex ddPCRTM assays / George Karlin-Neumann, Bin Zhang, and Claudia Litterst -- Quantification of Ccirculating microRNAs by droplet digital PCR / Manuela Ferracin and Massimo Negrini -- Droplet digital PCR for absolute quantification of extracellular microRNAs in plasma and serum : quantification of the cancer biomarker hsa-miR-141 / Maria D. Giraldez, John R. Chevillet, and Muneesh Tewari -- Droplet digital PCR next-generation sequencing library QC assay / Nicholas J. Heredia -- Phasing DNA markers using digital PCR / John Regan and George Karlin-Neumann -- ddTRAP : a method for sensitive and precise quantification of telomerase activity / Andrew T. Ludlow, Dawne Shelton, Woodring E. Wright, and Jerry W. Shay -- Highly efficient and reliable DNA aptamer selection using the partitioning capabilities of ddPCR : the Hi-Fi SELEX method ./ Aaron Ang, Eric Ouellet, Karen C. Cheung, and Charles Haynes.
Summary:
This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences.".
Series:
Springer protocols, 1949-2448 Methods in molecular biology, 1064-3745 ; 1768
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.