Generation of mutants of nuclear-encoded plastid proteins using CRISPR/Cas9 in the diatom Phaeodactylum tricornutum / Guillaume Allorent, Erika Guglielmino, Cécile Giustini, and Florence Courtois. Complex endosymbioses I : from primary to complex plastids, multiple independent events / Zoltán Füssy and Miroslav Oborník -- Complex endosymbioses II : the nonphotosynthetic plastid of apicomplexa parasites (The apicoplast) and its integrated metabolism / Cyrille Y. Botté and Yoshiki Yamaryo-Botté -- Diversity and plasticity of plastids in land plants / Katalin Solymosi, Johanna Lethin, and Henrik Aronsson -- Main functions of plastids / Norbert Rolland, Imen Bouchnak, Lucas Moyet, Daniel Salvi, and Marcel Kuntz -- Plastid transient and stable interactions with other cell compartments / Stefanie J. Mueller-Schuessele and Morgane Michaud -- Imaging plastids in 2D and 3D : confocal and electron microscopy Serena Flori, Pierre-Henri Jouneau, Benoit Gallet, Leandro F. Estrozi, Christine Moriscot, Guy Schoehn, Giovanni Finazzi, and Denis Falconet -- Purification of chloroplasts and chloroplast subfractions : envelope, thylakoids, and stroma-from spinach, pea, and Arabidopsis thaliana / Maryse A. Block and Catherine Albrieux -- Isolation of inner and outer membranes of the chloroplast envelope from spinach and pea / Maryse A. Block -- Purification of nongreen plastids (proplastids and amyloplasts) from angiosperms, and isolation of their envelope membranes / Claude Alban and Etienne-Pascal Journet -- In vitro protein import into isolated chloroplasts / Maryse A. Block -- Analysis of the MTL supercomplex at contact sites between mitochondria and plastids / Morgane Michaud -- Isolation of plastid fractions from the diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum Alexander F. Schober, Serena Flori, Giovanni Finazzi, Peter G. Kroth, and Carolina Río Bártulos -- Isolating the Plasmodium falciparum apicoplast using magnetic beads / Cyrille Y. Botté, Geoffrey I. McFadden, and Yoshiki Yamaryo-Botté -- Extraction and quantification of lipids from plant or algae / Valérie Gros, Josselin Lupette, and Juliette Jouhet -- Quantitative assessment of the chloroplast lipidome / Valérie Gros and Juliette Jouhet -- Determination of the DNA/RNA-associated subproteome from chloroplasts and other plastid types / Maha Chieb, Monique Liebers, Fabien Chevalier, Silva Lerbs-Mache, Robert Blanvillain, and Thomas Pfannschmidt -- Mapping plastid transcript population by circular reverse transcription polymerase chain reaction / Florence Courtois and Livia Merendino -- Bioinformatic analysis of chloroplast gene expression and RNA posttranscriptional maturations using RNA sequencing Bastien Malbert, Guillem Rigaill, Veronique Brunaud, Claire Lurin, and Etienne Delannoy -- Guide to the chloroplast transcriptome analysis using RNA-Seq / Elena J. S. Michel, Amber M. Hotto, Susan R. Strickler, David B. Stern, and Benoît Castandet -- Toolkit for the characterization of the photoprotective capacity of green algae / M. Águila Ruiz-Sola and Dimitris Petroutsos -- Rescue of deletion mutants to isolate plastid transformants in higher plants / Mohammad El Hajj, Mohd Fahdli Bin Hamdan, Elena Martin Avila, and Anil Day -- Genetic analysis of chloroplast biogenesis, and function and mutant collections / Sho Fujii, Hajime Wada, and Koichi Kobayashi -- Generation of mutants of nuclear-encoded plastid proteins using CRISPR/Cas9 in the diatom Phaeodactylum tricornutum / Guillaume Allorent, Erika Guglielmino, Cécile Giustini, and Florence Courtois.
Series:
Methods in molecular biology, 1064-3745 ; v. 1829 Springer protocols, 1949-2448
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