Screening the impact of sirtuin inhibitors on inflammatory and innate immune responses of macrophages and in a mouse model of endotoxic shock / Eleonora Ciarlo and Thierry Roger. Detection of sumo modification of endogenous histone deacetylase 2 (HDAC2) in mammalian cells / Simona Citro and Susanna Chiocca -- Analysis of epigenetic regulation of hypoxia-induced epithelial-mesenchymal transition in cancer cells by quantitative chromatin immunoprecipitation of histone deacetylase 3 (HDAC3) / Jian-Qiu Wang, Min-Zu Wu, and Kou-Juey Wu -- Molecular and functional characterization of histone deacetylase 4 (HDAC4) / Lin Li and Xiang-Jiao Yang -- Approaches for studying the subcellular localization, interactions, and regulation of histone deacetylase 5 (HDAC5) / Amanda J. Guise and Ileana M. Cristea -- Analysis of expression and functions of histone deacetylase 6 (HDAC6) / Miao Li, Yan Zhuang, and Bin Shan -- Analysis of histone deacetylase 7 (HDAC7) Aaternative splicing and its role in embryonic stem cell differentiation toward smooth muscle lineage / Junyao Yang, Andriana Margariti, and Lingfang Zeng -- Large-scale overproduction and purification of recombinant histone deacetylase 8 (HDAC8) from the human-pathogenic flatworm Schistosoma mansoni / Martin Marek ... [et al.] -- Visualization of HDAC9 spatiotemporal subcellular localization in primary neuron cultures / Noriyuki Sugo and Nobuhiko Yamamoto -- Expression and function of histone deacetylase 10 (HDAC10) in B cell malignancies / John Powers ... [et al.] -- Functional analysis of histone deacetylase 11 (HDAC11) / Jie Chen ... [et al.] -- Sirtuin1 (SIRT1) in the Acetylation of Downstream Target Proteins / Ana R. Gomes ... [et al.] -- Protocols for cloning, expression, and functional analysis of sirtuin2 (SIRT2) / Shaoping Ji , J. Ronald Doucette, and Adil J. Nazarali -- Cloning and characterization of sirtuin3 (SIRT3) / Andy W. C. Man, Bo Bai, and Yu Wang -- Identification of sirtuin4 (SIRT4) protein interactions : uncovering candidate acyl-modified mitochondrial substrates and enzymatic regulators / Rommel A. Mathias, Todd M. Greco, and Ileana M. Cristea -- Generation and purification of catalytically active recombinant sirtuin5 (SIRT5) protein / Surinder Kumar and David B. Lombard -- Sirtuin 6 (SIRT6) activity assays / Minna Rahnasto-Rilla, Maija Lahtela-Kakkonen, and Ruin Moaddel -- Molecular, cellular, and physiological characterization of sirtuin 7 (SIRT7) / Jiyung Shin and Danica Chen -- HDAC inhibitors / Heidi Olzscha ... [et al.] -- Assessment of the antiproliferative activity of a BET bromodomain inhibitor as single agent and in combination in non-hodgkin lymphoma cell lines / Elena Bernasconi ... [et al.] -- Screening the impact of sirtuin inhibitors on inflammatory and innate immune responses of macrophages and in a mouse model of endotoxic shock / Eleonora Ciarlo and Thierry Roger.
Summary:
This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone Deacetylases: Methods and Protocols is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Histone Deacetylases: Methods and Protocols, is a valuable resource for investigators working on epigenetics, molecular biology, and genetics.
Series:
Methods in molecular biology, 1064-3745 ; 1436 Springer protocols, 1949-2448
This resource is supported by the Institute of Museum and Library Services under the provisions of the Library Services and Technology Act as administered by State Library of Iowa.