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Title:

RNAi and small regulatory RNAs in stem cells : methods and protocols / edited by Baohong Zhang.

Publisher:

Humana Press ;

Copyright Date:

2017

Description:

xi, 311 pages : illustrations (some color) ; 26 cm

Subject:

RNA.
Laboratory manuals.

Other Authors:

Zhang, Baohong, editor.

Notes:

Includes bibliographical references and index.

Contents:

CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells / Yinghua Wang, Yifu Ding, and Jinsong Li. RNAi and MicroRNA-mediated gene regulation in stem sells / Alexander K. Murashov -- Forward RNAi screens in human hematopoietic stem cells / Roman Galeev, Christine Karlsson, Aurélie Baudet, and Jonas Larsson -- Silencing of ATP11B by RNAi-induced changes in neural stem cell morphology / Jiao Wang, Qian Wang, Fangfang Zhou, Dong Wang, and Tieqiao Wen -- High-efficiency transfection of glioblastoma cells and a simple spheroid migration assay / Carsten Hagemann, Diana Amend, Almuth F. Kessler, Thomas Linsenmann, Ralf-Ingo Ernestus, and Mario Löhr -- Construction and application of random dsRNA interference library for functional genetic screens in embryonic stem cells / Xiaoxing Cheng and Rui Jian -- Efficient depletion of essential gene products for loss-of-function studies in embryonic stem cells / Soizik Berlivet, Isabelle Hmitou, Hélène Picaud, and Matthieu Gérard -- Regulation and/or repression of cholinergic differentiation of murine embryonic stem cells using RNAi directed against transcription factor L3/Lhx8 / Takayuki Manabe and Akio Wanaka -- Recessive genetic screen for components of the RNA interference pathway performed in mouse embryonic stem cells / Melanie I. Trombly and Xiaozhong Wang -- RNAi knockdown of Ape1 gene in the differentiation of mouse embryonic stem cells / Gang-Ming Zou, Jieqing Yu, Cynthia LeBron, and Yumei Fu -- Efficient transfection method for differentiation and cell proliferation of mouse embryonic stem cells / Jun-Yang Liou, Bor-Sheng Ko, and Tzu-Ching Chang -- Implanting glioblastoma spheroids into rat brains and monitoring tumor growth by MRI volumetry / Mario Löhr, Thomas Linsenmann, Anna Jawork, Almuth F. Kessler, Nils Timmermann, György A. Homola, Ralf-Ingo Ernestus, and Carsten Hagemann -- RNAi-based techniques for the analysis of gene function in Drosophila germline stem cells / Amelia J. Blake, Danielle S. Finger, Victoria L. Hardy, and Elizabeth T. Ables -- In vivo RNAi in the Drosophila follicular epithelium : analysis of stem cell maintenance, proliferation, and differentiation / Veit Riechmann -- Phenotype-based RNAi screening for Ras-ERK/MAPK signaling-associated stem cell regulators in C. elegans / Myon-Hee Lee and Dong Suk Yoon -- Engineering human mesenchymal stem cells to release adenosine using miRNA technology / Gaoying Ren and Detlev Boison -- Efficient gene knockdowns in mouse embryonic stem cells using MicroRNA-based shRNAs / Jianlong Wang -- Using quantitative real-time PCR to detect MicroRNA expression profile during embryonic stem cell differentiation / Xiaoping Pan, Alexander K. Murashov, Edmund J. Stellwag, and Baohong Zhang -- Genetic tools for self-organizing culture of mouse embryonic stem cells via small regulatory RNA-mediated technologies, CRISPR/Cas9, and inducible RNAi / Nozomu Takata, Eriko Sakakura, Tetsushi Sakuma, and Takashi Yamamoto -- CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells / Yinghua Wang, Yifu Ding, and Jinsong Li.

Series:

Methods in molecular biology, 1064-3745 ; volume 1622
Springer protocols, 1949-2448

ISBN:

1493971069
9781493971060

OCLC:

(OCoLC)978289943

LCCN:

2017944281

Locations:

OVUX522 -- University of Iowa Libraries (Iowa City)