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Title:
Cytokine bioassays : methods and protocols / edited by Ivana Vancurova.
Publisher:
Humana Press ;
Copyright Date:
c2014
Description:
xiv, 367 p. : ill. (some col.) ; 26 cm.
Subject:
Cytokines--Laboratory manuals.
Biological assay--Laboratory manuals.
Biological Assay
Cytokines
Other Authors:
Vancurova, Ivana, editor.
Notes:
Includes bibliographical references and index.
Contents:
Assessment of cytokine-modulated proteasome activity / Christopher J. Kirk, Saul R. Powell, and Edmund J. Miller -- Cytokine-induced neutrophil chemotaxis assay / Dennis Davidson and Hardik Patel -- In vitro one-dimensional assay to study growth factor-regulated tumor cell-macrophage interaction / Ved P. Sharma, Brian T. Beaty, Dianne Cox, John S. Condeelis, and Robert J. Eddy -- Chemotactic responses by macrophages to a directional source of a cytokine delivered by a micropipette / Michael Cammer and Dianne Cox -- Assessment of phagocytic activity of cultured macrophages using fluorescence microscopy and flow cytometry / Lokesh Sharma ... [e tal.] -- Assessment of cytokine-modulated proteasome activity / Christopher J. Kirk, Saul R. Powell, and Edmund J. Miller --
Use of shRNA for stable suppression of chemokine receptor expression and function in human cancer cell lines / Nicole Salazar ... [et al.] -- Mix-and-measure assay for determining the activation status of endogenous Cdc42 in cytokine-stimulated macrophage cell lysates / Veronika Miskolci ... [et al] -- Analysis of the cell surface expression of cytokine receptors using the surface protein biotinylation method / Mahmud Arif Pavel -- Detection of CXCR2 cytokine receptor surface expression using immunofluorescence / Clarissa Lam ... [et al.] -- Detecting Tie2, an endothelial growth factor receptor, by using immunohistochemistry in mouse lungs / Prajna P. Guha, Sascha A. David, and Chandra C. Ghosh -- Use of shRNA for stable suppression of chemokine receptor expression and function in human cancer cell lines / Nicole Salazar ... [et al.] --
Quantitative analysis of bortezomib-induced IL-8 gene expression in ovarian cancer cells / Bipradeb Singha ... [et al.] -- Cytokine detection by flow cytometry / Jian-Ge Qiu ... [et al.] -- Analysis of IL-17 production by flow cytometry and ELISPOT assays / Ling Zhao ... [et al] -- Interleukin-1 (IL-1) immunohistochemistry assay in oral squamous cell carcinoma / Takaaki Kamatani -- Immunofluorescence and subsequent confocal microscopy of intracellular TNF in human neutrophils / Janet Rollins and Veronika Miskolci -- Evaluating cytoplasmic and nuclear levels of inflammatory cytokines in cancer cells by western blotting / Himavanth R. Gatla ... [et al.] -- Western analysis of intracellular interleukin-8 in human mononuclear leukocytes / Veronika Miskolci ... [et al.] -- Quantitative analysis of bortezomib-induced IL-8 gene expression in ovarian cancer cells / Bipradeb Singha ... [et al.] --
Radiolabeled semi-quantitative RT-PCR assay for the analysis of alternative splicing of interleukin genes / Felitsiya Shakola ... [et al.]. Chromatin immunoprecipitation analysis of bortezomib- mediated inhibition of NFκB recruitment to IL-1β and TNFα gene promoters in human macrophages / Shannon Sanacora, Tzu-Pei Chang, and Ivana Vancurova -- Analysis of TGFβ1 and IL-10 transcriptional regulation in CTCL cells by chromatin immunoprecipitation / Tzu-Pei Chang, Myra Kim, and Ivana Vancurova -- Radiolabeled semi-quantitative RT-PCR assay for the analysis of alternative splicing of interleukin genes / Felitsiya Shakola ... [et al.].
Series:
Methods in molecular biology, 1064-3745 ; 1172
Springer protocols, 1949-2448
ISBN:
9781493909278
1493909274
OCLC:
(OCoLC)874223371
Locations:
OVUX522 -- University of Iowa Libraries (Iowa City)

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