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05691aam a2200349 i 4500 001 E7CA9B78EAD311E7BA71765497128E48 003 SILO 005 20171227010214 008 160203t20162016nyua b 001 0deng d 010 $a 2016933776 020 $a 9781493936656 020 $a 1493936654 035 $a (OCoLC)936532711 040 $a YDXCP $b eng $e rda $c YDXCP $d BTCTA $d OCLCQ $d IDL $d OCLCO $d OCLCF $d NUI $d UtOrBLW $d SILO 245 00 $a Histone deacetylases : $b methods and protocols / $c edited by Sibaji Sarkar. 264 1 $a New York : $b Humana Press, $c [2016] 300 $a xii, 337 pages : $b illustrations (some color) ; $c 26 cm. 490 1 $a Methods in molecular biology, $x 1064-3745 ; $v 1436 490 1 $a Springer protocols, $x 1949-2448 504 $a Includes bibliographic references and index. 505 00 $t Screening the impact of sirtuin inhibitors on inflammatory and innate immune responses of macrophages and in a mouse model of endotoxic shock / $r Eleonora Ciarlo and Thierry Roger. $t Detection of sumo modification of endogenous histone deacetylase 2 (HDAC2) in mammalian cells / $r Simona Citro and Susanna Chiocca -- $t Analysis of epigenetic regulation of hypoxia-induced epithelial-mesenchymal transition in cancer cells by quantitative chromatin immunoprecipitation of histone deacetylase 3 (HDAC3) / $r Jian-Qiu Wang, Min-Zu Wu, and Kou-Juey Wu -- $t Molecular and functional characterization of histone deacetylase 4 (HDAC4) / $r Lin Li and Xiang-Jiao Yang -- $t Approaches for studying the subcellular localization, interactions, and regulation of histone deacetylase 5 (HDAC5) / $r Amanda J. Guise and Ileana M. Cristea -- $t Analysis of expression and functions of histone deacetylase 6 (HDAC6) / $r Miao Li, Yan Zhuang, and Bin Shan -- $t Analysis of histone deacetylase 7 (HDAC7) Aaternative splicing and its role in embryonic stem cell differentiation toward smooth muscle lineage / $r Junyao Yang, Andriana Margariti, and Lingfang Zeng -- $t Large-scale overproduction and purification of recombinant histone deacetylase 8 (HDAC8) from the human-pathogenic flatworm Schistosoma mansoni / $r Martin Marek ... [et al.] -- $t Visualization of HDAC9 spatiotemporal subcellular localization in primary neuron cultures / $r Noriyuki Sugo and Nobuhiko Yamamoto -- $t Expression and function of histone deacetylase 10 (HDAC10) in B cell malignancies / $r John Powers ... [et al.] -- $t Functional analysis of histone deacetylase 11 (HDAC11) / $r Jie Chen ... [et al.] -- $t Sirtuin1 (SIRT1) in the Acetylation of Downstream Target Proteins / $r Ana R. Gomes ... [et al.] -- $t Protocols for cloning, expression, and functional analysis of sirtuin2 (SIRT2) / $r Shaoping Ji , J. Ronald Doucette, and Adil J. Nazarali -- $t Cloning and characterization of sirtuin3 (SIRT3) / $r Andy W. C. Man, Bo Bai, and Yu Wang -- $t Identification of sirtuin4 (SIRT4) protein interactions : uncovering candidate acyl-modified mitochondrial substrates and enzymatic regulators / $r Rommel A. Mathias, Todd M. Greco, and Ileana M. Cristea -- $t Generation and purification of catalytically active recombinant sirtuin5 (SIRT5) protein / $r Surinder Kumar and David B. Lombard -- $t Sirtuin 6 (SIRT6) activity assays / $r Minna Rahnasto-Rilla, Maija Lahtela-Kakkonen, and Ruin Moaddel -- $t Molecular, cellular, and physiological characterization of sirtuin 7 (SIRT7) / $r Jiyung Shin and Danica Chen -- $t HDAC inhibitors / $r Heidi Olzscha ... [et al.] -- $t Assessment of the antiproliferative activity of a BET bromodomain inhibitor as single agent and in combination in non-hodgkin lymphoma cell lines / $r Elena Bernasconi ... [et al.] -- $t Screening the impact of sirtuin inhibitors on inflammatory and innate immune responses of macrophages and in a mouse model of endotoxic shock / $r Eleonora Ciarlo and Thierry Roger. 520 $a This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone Deacetylases: Methods and Protocols is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Histone Deacetylases: Methods and Protocols, is a valuable resource for investigators working on epigenetics, molecular biology, and genetics. 650 0 $a Histone deacetylase. $0 http://id.loc.gov/authorities/subjects/sh00007235 650 12 $a Histone Deacetylases. $0 https://id.nlm.nih.gov/mesh/D006655 655 2 $a Laboratory Manuals. $0 https://id.nlm.nih.gov/mesh/D020484 700 1 $a Sarkar, Sibaji, $e writer of preface. $e writer of preface. $0 http://id.loc.gov/authorities/names/nb2016013570 773 18 $w 990001282000202771 $g v:1436 830 0 $a Methods in molecular biology (Clifton, N.J.) ; $v v. 1436. $x 1064-3745 $0 http://id.loc.gov/authorities/names/n92002874 830 0 $a Springer protocols (Series). $x 1949-2448 $0 http://id.loc.gov/authorities/names/no2008180772 941 $a 1 952 $l OVUX522 $d 20180710101830.0 956 $a http://locator.silo.lib.ia.us/search.cgi?index_0=id&term_0=E7CA9B78EAD311E7BA71765497128E48Initiate Another SILO Locator Search